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Standard Operating Procedures
SOP Wiki (lab members only): saleslab.shoutwiki.com
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Instrumentation
- General
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Eppendorf Centrifuge 5430R
Water distiller
Innova 44R shaking incubator
For culturing purposes our lab is equipped with two Innova 44R shaking incubators and one incubation chamber for photosynthetic growth.
- Deionized water is provided in two quality grades by either a distiller (a) or a reverse osmosis filtration system (not shown).
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Shimadzu HPLC
Measurement
- The lab has a Shimadzu HPLC system equipped with a UV-Vis and conductivity meter with columns for cation and anionchromatography as well as reverse-phase chromatography. Anion chromatography may be enhanced by the use of a CARS (Continuous Anion Regeneration System) setup. To analyze total organic carbon, inorganic carbon, and total nitrogen content of aqueous samples a Shimadzu TOC/TN analyzer with integrated autosampler is used. For measurement of dissolved gases , a Shimadzu GC-2010 Plus high-end gas chromatograph is employed.
- All chromatography instruments share one exclusive terminal and are operated by LabSolutions software.
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Shimadzu TOC/TN analyzer
Shimadzu GC-2010 Plus gas chromatographer
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- Molecular work
- We have a Roche LightCycler 480 real-time PCR system for quantitative PCR (qPCR) and a BIORAD iCycler thermocycler and an Eppendorf Thermomixer for general molecular biology use. The laboratory also has a Nanodrop 1000 for measuring DNA, RNA and protein concentrations.
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Fisher Scientific PCR workstation
Bio-Rad iCycler: Real-Time PCR Detection System
Roche LightCycler: Real-Time PCR system
Qiagen QIAcube: Purification of DNA, RNA and proteins
Qubit 2.0 Fluorometer: DNA & RNA quantification
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